APC anti-mouse H-2Kb Antibody anti-H-2Kb - AF6-88.5 (2024)

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Product Details Antigen Details Documentation Reviews Related Protocols Related Products Pathways Pages Related FAQs Other Formats Certificate of Analysis Customers Also Purchased Biotin anti-mouse H-2Kb FITC anti-mouse H-2Kb PE anti-mouse H-2Kb Purified anti-mouse H-2Kb Alexa Fluor® 488 anti-mouse H-2Kb Alexa Fluor® 647 anti-mouse H-2Kb Pacific Blue™ anti-mouse H-2Kb PerCP/Cyanine5.5 anti-mouse H-2Kb APC anti-mouse H-2Kb PE/Cyanine7 anti-mouse H-2Kb Alexa Fluor® 700 anti-mouse H-2Kb Brilliant Violet 510™ anti-mouse H-2Kb Brilliant Violet 421™ anti-mouse H-2Kb
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Version: 1 Revision Date: 11/30/2012

  • Product Details
  • Antigen Details
  • Documentation
  • Reviews
  • Related Protocols
  • Related Products
  • Related FAQs
  • Other Formats
  • Certificate of Analysis

Clone
AF6-88.5 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (BALB/c) IgG2a, κ
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Product Citations
publications
APC anti-mouse H-2Kb Antibody anti-H-2Kb - AF6-88.5 (1)
  • APC anti-mouse H-2Kb Antibody anti-H-2Kb - AF6-88.5 (2)

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Cat # Size Price Quantity Check Availability Save
116517 25 µg £65
116518 100 µg £179

Description

The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kb haplotype. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The AF6-88.5 antibody does not cross-react with other haplotypes (d, f, j, k, p, q, r, s, u, v).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C57BL mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml

Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application
FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunohistochemical staining of acetone-fixed frozen sections3. Clone AF6-88.5 is not suitable for immunohistochemical staining of formalin-fixed paraffin embedded sections.

Does not cross-react with other haplotypes (e.g., d, f, j, k, p, q, r, s, u, v).

Application References

(PubMed link indicates BioLegend citation)

  1. Loken MR, et al. 1982. J. Immunol. Methods 50:R85.
  2. Wall KA, et al. 1983. J. Immunol. 131:1056.
  3. Andersson M, et al. 1998. J. Immunol. 161:6475.
  4. Shao H, et al. 2005. J. Immunol. 175:1851.
  5. Hui S, et al. 2005. J. Immunol. 175:1851. PubMed
  6. Zhou K, et al. 2010. Cytotherapy. 12:735. PubMed
  7. Desvignes L, et al. 2012. J Immunol. 188:6205. PubMed
Product Citations
  1. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  2. Sethumadhavan S, et al. 2017. PLoS One. . 10.1371/journal.pone.0187314. PubMed
  3. Regev O, et al. 2022. Front Immunol. 13:849701. PubMed
  4. Pierini S, et al. 2021. J Immunother Cancer. 9:. PubMed
  5. Ferrari de Andrade L, et al. 2020. Cancer Immunol Res. 0.867361111. PubMed
  6. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  7. Kremenovic M, et al. 2022. J Immunother Cancer. 10:. PubMed
  8. Gruber T, et al. 2020. JCI Insight. 5:00. PubMed
  9. Perry JSA, et al. 2018. Immunity. 48:923. PubMed
  10. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  11. Wang X, et al. 2011. Blood. 118:6426. PubMed
  12. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  13. Canton J, et al. 2021. Nat Immunol. 22:140. PubMed
  14. Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed
  15. Gurusamy D, et al. 2020. Cancer Cell. 37(6):818-833.e9. PubMed
  16. Oladiran O, et al. 2021. J Neuroinflammation. 77:18. PubMed
  17. Sheng W et al. 2018. Cell. 174(3):549-563 . PubMed
  18. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  19. Kalbasi A, et al. 2020. Sci Transl Med. :12. PubMed
  20. Fujimoto K, et al. 2022. Sci Rep. 12:18313. PubMed
  21. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  22. Bagati A, et al. 2020. Cancer Cell. 39(1):54-67.e9. PubMed
  23. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  24. Yoshida S, et al. 2022. PLoS One. 17:e0264317. PubMed
  25. Freed-Pastor WA, et al. 2021. Cancer Cell. 39:1342. PubMed
  26. Gejman RS et al. 2018. eLife. 7 pii: e41090. PubMed
RRID
AB_10568693 (BioLegend Cat. No. 116517)
AB_10564404 (BioLegend Cat. No. 116518)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kb haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Review Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Review Immunol. 16:323.
3. York I, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kb on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

  • Cell Surface Flow Cytometry Staining Protocol

Related Products

Description Clone Applications

Pathways

  • Cancer Immunoediting
  • CD4 and CD8 T-Cell Lineage
  • Immunologic Networks 2011
  • Regulatory T Cells

Pages

  • Quality Control
  • Multicolor Staining Guide
  • Fluorescence Spectra Analyzer
  • Fluorophore Brightness Index
  • Custom Panel Construction
  • Fluorophore Equivalency Tool

Related FAQs

Other Formats

View All H-2Kb Reagents Request Custom Conjugation

Description Clone Applications
Biotin anti-mouse H-2Kb AF6-88.5 FC
FITC anti-mouse H-2Kb AF6-88.5 FC
PE anti-mouse H-2Kb AF6-88.5 FC
Purified anti-mouse H-2Kb AF6-88.5 FC,IHC-F,IP
Alexa Fluor® 488 anti-mouse H-2Kb AF6-88.5 FC
Alexa Fluor® 647 anti-mouse H-2Kb AF6-88.5 FC
Pacific Blue™ anti-mouse H-2Kb AF6-88.5 FC
PerCP/Cyanine5.5 anti-mouse H-2Kb AF6-88.5 FC
APC anti-mouse H-2Kb AF6-88.5 FC
PE/Cyanine7 anti-mouse H-2Kb AF6-88.5 FC
Alexa Fluor® 700 anti-mouse H-2Kb AF6-88.5 FC
Brilliant Violet 510™ anti-mouse H-2Kb AF6-88.5 FC
Brilliant Violet 421™ anti-mouse H-2Kb AF6-88.5 FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse H-2Kb

    C57BL/6 mouse splenocytes stained with biotinylated AF6-88.5...

  • FITC anti-mouse H-2Kb

    C57BL/6 mouse splenocytes stained with AF6-88.5 FITC

  • PE anti-mouse H-2Kb

    C57BL/6 mouse splenocytes stained with AF6-88.5 PE

  • Purified anti-mouse H-2Kb

    C57BL/6 mouse splenocytes stained with purified AF6-88.5, fo...

  • Alexa Fluor® 488 anti-mouse H-2Kb

    C57BL/6 mouse splenocytes stained with AF6-88.5 Alexa Fluor®...

  • Alexa Fluor® 647 anti-mouse H-2Kb

    C57BL/6 mouse splenocytes stained with AF6-88.5 Alexa Fluor®...

  • Pacific Blue™ anti-mouse H-2Kb

  • PerCP/Cyanine5.5 anti-mouse H-2Kb

    C57BL/6 splenocytes stained with AF6-88.5 PerCP/Cyanine5.5

  • APC anti-mouse H-2Kb

    C57BL/6 mouse splenocytes stained with AF6-88.5 APC

  • PE/Cyanine7 anti-mouse H-2Kb

    C57BL/6 mouse splenocytes were stained with PE/Cyanine7 AF6-...

  • Alexa Fluor® 700 anti-mouse H-2Kb

    C57BL/6 mouse spenocyte were stained with H-2Kb (...

  • Brilliant Violet 510™ anti-mouse H-2Kb

    C57BL/6 mouse splenocytes were stained with H-2Kb (clone AF6...

  • Brilliant Violet 421™ anti-mouse H-2Kb

    C57BL/6 mouse splenocytes were stained with H-2Kb...
APC anti-mouse H-2Kb Antibody anti-H-2Kb - AF6-88.5 (2024)
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